Liposome Calculator for Pc

1. The user should follow the simple rule to ensure proper functionality: all known data should be provided in the following order (Only yellow fields are adjustable): solution or solute nicknames(optional)-> Mw (if needed)-> initial concentrations (must!)-> volumes (if known)-> molar ratios (if known)-> Total Molarity (if known)-> Total Volume (if known).

2. For example: final molar concentration and molar ratios of the components can be adjusted at any time if molar concentrations of starting solutions and final suspension volume are provided.

3. Components concentrations (molarity and mg/ml) and moles, and volumes of stock solutions are calculated based upon data provided, such as: initial concentrations of the stock solutions, and required molar ratios.

4. Second is Analyzer that helps in analysis of liposome parameters, such as number of molecules per vesicle, inner, entrapped volume and encapsulation.

5. Watch out the molar ratios to ensure that all starting components are included! Program will expel components for which insufficient data is provided! Thus, for example concentration in g/l cannot be included in calculations if Mw of solute is not provided.

6. Number of Molecules: Average number of lipid molecules in a single liposome (vesicle).

7. Inner Volume: Average volume entrapped in a single liposome.

8. Encapsulation Efficiency: In this context is represented by ratio of the total entrapped volume to total solution volume.

9. Number of Liposomes: Average total number of liposomes in the analyzed solution.

10. Stock solutions of lipids (or any other compound) are mixed in appropriate molar ratios.

11. Automatically uploaded from Liposome Calculator activity and can be corrected at any time.

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