Isoelectric Point for Pc

1. If peptide is analysed, then tapping “Analyse” button, calculates the molar weight, counts and fills in the number of recognized acidic and basic residues into the appropriate cells in the amino acid info table.

2. Application provides data for most common acidic and basic amino acid residues, but every cell can be updated! Sequence analysis reverts the table back to its original state.

3. Long press on 'Reset' button removes amino acid data from table if the analysed molecule is not based on amino acid sequence or alternatively brings it back if peptide is analysed.

4. Isoelectric Point (IEP) helps to estimate the charge of macromolecule (proteins, peptides, amino acids, DNA, etc.), consisting of basic and acidic residues as a function of pH value of solution.

5. The cells are colored according to the data input allowed (unchangeable): red for acidic and blue for basic function.

6. If user updates any of the data cells, the program will not start to plot until user press calculate button and only if the update is accepted.

7. Important! Table data can be fully customized after or without any sequence analysis! IEP calculation uses table values, whatever their origin is .

8. The “Calculate” button initiates the analysis of the table data and outputs the IEP value.

9. The application graphically represents the charge fluctuation over the whole range of pH values and finds out the isoelectric point value.

10. Simple press on 'Chain' button launches chain picker dialog if multiple chains are defined in FASTA file.

11. Cells for acidic residues are coloured red and for basic are blue.

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